hisS [2019-03-30 18:21:37]

histidyl-tRNA synthetase

locus: BSU27560

pI: 5.23

mw: 47.97 kDa

protein length: 424 aa

gene length: 1275 bp

function: translation

product: histidyl-tRNA synthetase

essential: yes

ec: 6.1.1.21

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,816,535 2,817,809

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His) (according to Swiss-Prot)

Protein family

• class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Structure

• [PDB|1H4V] (from ''Thermus thermophilus'', 43% identity, 61% similarity) [Pubmed|11399074]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|69C7EF7E76821FF3D9E866838B24C3866CA9C189|hisS]-[gene|2B61D17950ADE0247B0CF4AE33548E15C0DDCD9B|aspS]

  regulatory mechanism

  • [regulon|T-box|T-box]: transcriptional antitermination, via tRNA controlled [SW|RNA switch], in [regulon|T-box|T-box]

  regulation

  • induced by histidine or aspartate limitation ([SW|T-box]) [Pubmed|19258532]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

References

• 19258532